1) General description of the product
产品概况
The product we are looking for is a purified and sterilized protein that is produced using food based growth medium and fermented much like the making of beer, wine, yogurt or cheese.
我们正在寻找的是一种纯化的和无菌的蛋白质，以食品为基础的生长介质，像啤酒、葡萄酒、酸奶或奶酪那样发酵而成。
To make it is used a safe and benign naturally occurring microbe found ubiquitously on every continent of the world. The procedure include grow the fermentation then harvest the protein, remove the water, pelletize the protein in liquid nitrogen and freeze-dry it in a food processing plant.
为了得以很好的使用，它是一种安全的、良性的自然产生的微生物，广泛存在于世界的每一个角落。其过程包括发酵，然后收获蛋白质，脱水，在液氮中将蛋白质颗粒化，然后在食品加工厂将其冷冻干燥。
Then a measured amount of the product protein is packed into hermetically sealed bags, ten of those bags are then placed in a box and the boxes are then sent to a medical sterilization plant to have the box, bags and the protein within sterilized to make the shelf life last longer.
然后，将一定量的蛋白质包装在密封的袋子中，每10个袋子放在一个盒子里，然后将这些盒子送往医疗消毒厂，对盒子、袋子和里面的蛋白质进行消毒处理，以延长保质期。
2) Supplier field
供应商领域
All kind of companies that are capable of doing fermentation processes. Such companies can be beer breweries, pharmaceutical producers, and companies that generally work in the breeding of bacteria. Products of such companies can be beer, flavor enhancers for Energy Drinks (generally sugar free flavor enhancers are often produced by certain bacteria), natural colorings for foods, aerosol to sterilize mosquitoes, or carpet of algae that neutralize oil in the seawater in case of accidents.
所有能够进行发酵过程的公司。这类公司可以是啤酒酿造商、制药生产商、以及一般从事细菌培育的公司。这些公司的产品可以是啤酒、能源饮料的调味剂（通常是由某些细菌生产的无糖调味剂）、食品的天然色素、驱蚊剂、或者是在发生事故时能中和海水和油的海藻地毯。
3) Supplier capabilities/equipment
供应商功能/设备
-Fully equipped Analytical Laboratory
设备齐全的分析实验室
-Media sterilization system
媒体消毒系统
-Media weighing system
媒体称重系统
-Shake Flasks
摇瓶
-Incubators
孵化器
-300L seed fermenter
300 升种子发酵罐
-10,000 L intermarry fermenter
1万升的互助式发酵罐
-20-30,000L production fermenter
2-3万升生产发酵罐
-Large Steam Generator
大型蒸汽发生器
-Large Cooling System with precise controls
有精确控件的大型冷却系统
-Off gas monitoring system
废气监测系统
-Acid and caustic feed systems
酸性和腐蚀性供料系统
-Cell Removal System, plate and frame or cartridge style preferred over centrifuge
细胞清除系统，板和框或墨盒式优先于离心机
-5-10,000L jacketed holding tank
5-10000升夹套式收集罐
-Liquid Nitrogen Storage Tank preferable but not mandatory
液氮储罐(最好有，但是不是必需的)
-Liquid Nitrogen Bath palletization system preferable but not mandatory
液氮托盘系统(最好有，但是不是必需的)
-Large Walk-in freezer preferable but not mandatory / can be rented during production time if not available on site
大型步入式冰箱（最好有，但是不是必需的/在生产期间，如果在现场没有的话，可以租赁）
-1000L insulated storage bins
1000升绝缘储存箱
-Proximity to a Freeze Drying service
接近冷冻干燥服务
-Proximity to Packaging service
接近包装服务
-Proximity to Sterilization service
接近消毒服务
-Proximity to a large commercial storage freezer
靠近大型商业冷藏库
The protein is gram negative, the fermentation plants must be able to have gram negative production
蛋白质为革兰氏阴性，发酵植物必须能进行革兰氏阴性生产。
4) Production process
生产过程
1. Incubate shake flask fermentation box containing a defined growth media with the organism.
2. Grow for a period of time
3. Transfer to the sterilized seed tank containing the same media.
4. Grow the seed tank at the appropriate temperature for a longer period.
5. Transfer to the sterilized Intermediary tank containing the same media.
6. Grow the Intermediary tank at the appropriate temperature for a defined period.
7. Transfer the contents of the Intermediary tank to the sterilized production tank; also containing the same media.
8. Grow at the appropriate temperature; controlling the pH to a very tight specification using acid & base.
9. At harvest; cool or heat to a precise temperature, then start concentrating the cell mass.
10. When concentration has been completed, send the broth to the liquid nitrogen bath.
11. Remove and store the frozen wet pellets in the 1000L insulated storage bins and keep frozen in the large walk-in freezer or transfer immediately to a large commercial storage freezer
1. 将摇瓶发酵箱与有机体一起孵育，其中含有特定的生长培养基。
2. 培育一段时间。
3. 转移到含有相同介质的灭菌种子罐中。
4. 在适当的温度下长时间培育灭菌种子罐。
5. 转移到含有相同介质的消毒中间罐中。
6. 将中间罐置于适当的温度下，培育一定的时间。
7. 将中间罐的内装物转移到同样含有相同介质的灭菌生产罐中。
8. 在适当的温度下进行培育；用酸和碱将pH控制在非常严格的范围内。
9. 收获时，冷却或加热到精确的温度，然后开始浓缩细胞群。
10. 浓缩完毕后，将液体培养基送至液氮盆中。
11. 取出并将冷冻湿粒料储存在1000升绝缘储存箱中，并将其保存在大型步入式冷冻机中或者立即将其运输安置于大型商业冷藏库。
5) Product Chemical Characterization
产品化学表征
- Protein (Bradford)
- Volatiles (105C)
- Ash (700 C)
- Carbohydrates (High Plasma Liquid Chromotography)
- Mono & Disaccharides
- Organic Acids (High Plasma Liquid Chromotography)
- Total Nitrogen (Kjeldahl)
- metals (Ion Coupled Plasma)
- Phosphate (Ionization Chromotography)
- Total Phosphate (Mo04)
- Sulfate (Ionization Chromotography)
- Chloride (Ionization Chromotography)
- Nucleic Acids
- 蛋白质(布拉德福德)
- 挥发物(105C)
- 灰分(700C)
- 碳水化合物(高等离子体液相色谱)
- 单双糖
- 有机酸(高等离子体液相色谱)
- 总氮(凯氏测氮法)
- 金属(离子耦合等离子体)
- 磷酸盐(电离显色术)
- 总磷酸盐(Mo04)
- 硫酸盐(电离显色术)
- 氯化物(电离显色术)
- 核酸
6) Supplier tasks and offer content
供应商的任务和供应内容
The supplier will be in charge of the following tasks:
1. Production of a sample quantity
2. Supply all raw material
3. Production process
4. Storage
供应商将负责下列工作：
1.样品量的产生
2.所有原材料供应
3.生产过程
4.储存
7) Quantity of production
产量
|
Sample production 2018
2018年样品生产
|
100 Kg
|
|
Production 2019
2019年生产
|
5-10 t
|
|
Production 2020-2022
2020-2022年生产
|
15-20 t
|
