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Specifications
- Host Species
- Rabbit
- Species Reactivity
- N/A
- Immunogen
- MASMTGGQQMG (T7) conjugated to KLH
- Conjugate
- Unconjugated
Applications
- Application Notes
- Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use highquality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Target
- Alternative Names
- 10 antibody; Major capsid protein 10A antibody; MASMTGGQQMG epitope tag antibody; MASMTGGQQMG tag antibody; Minor capsid protein 10B antibody; T7 epitope tag antibody; Rabbit Anti-T7 Epitope Tag ; Anti-T7 Epitope Tag ; T7 Epitope Tag Rabbit Anti-T7 Epito
References
Liu, QY; Wang, LF; et al. Expression and characterization of a novel human sperm membrane protein. BIOLOGY OF REPRODUCTION 54:323-330(1996).
Bassi, EJ; Vernal, J; et al. Expression, purification and immunodetection of a recombinant fragment (residues 179-281) of the G protein from rabies virus ERA strain. PROTEIN EXPRESSION AND PURIFICATION 59:309-313(2008).
