1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Stephen Van Dien
2 Gene expression Engineering. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Nicholas J. Morse and Hal S. Alper
3 Heterologous Pathway Engineering. . . . . . . . . . . . . . . . . . . . . . . . . 31
Wei Niu, Jiantao Guo and Steve Van Dien
4 Leveraging Gene Synthesis, Advanced Cloning Techniques,
and Machine Learning for metabolic Pathway Engineering. . . . . . . 53
Kedar G. Patel, Mark Welch and Claes Gustafsson
5 Tolerance of Microbial Biocatalysts to Feedstocks, Products,
and Environmental Conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Mian Huang, George Peabody and Katy C. Kao
6 Biobased Technology Commercialization: The importance
of Lab to Pilot Scale-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
James P. Wynn, Robert Hanchar, Susanne Kleff, David Senyk
and Tonya Tiedje
